Figure 8 | Scientific Reports

Figure 8

From: Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge

Figure 8

Detection of MOMP-specific antibodies after immunization with UV-inactivated EB in combination with different doses of VCG. Groups of chickens were immunized and boosted IN as described in the materials and methods section. Serum samples were collected from 5 chickens per group on days 7, 14 and 21 after the booster immunization as indicated in the Materials and Methods. MOMP-specific antibody titers were assessed by a MOMP-based antibody ELISA. Results are presented as the reciprocal of the highest serum dilution with an absorbance above the cut-off value (mean negative OD values plus 2 times the SD). The data are from one of two independent experiments with similar results. Significant differences between experimental groups were evaluated by One-way ANOVA with Tukey’s post multiple comparison test at p*** < 0.001, p** < 0.01 and p* < 0.05.

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