Figure 4

MB is a more effective ROS scavenger than Vitamin A and Vitamin C in human skin fibroblasts. (A) Young (Left) and old (Right) male fibroblasts proliferation during the fourteen-day treatment with control PBS, 0.01% DMSO, 100 nM MB, 100 nM Vitamin A, 100 μM Vitamin C, and 100 nM MB + 100 μM Vitamin C, respectively. p < 0.05, **p < 0.01 comparing with Control PBS on the same day; # p < 0.05 or ## p < 0.01 comparing with MB + Vitamin C-treated cells on the same day. (B) Young (Left) and old (Right) female fibroblasts proliferation during the fourteen-day treatment with control PBS, 0.01% DMSO, 100 nM MB, 100 nM Vitamin A, 100 μM Vitamin C, and 100 nM MB + 100 μM Vitamin C, respectively. p < 0.05, **p < 0.01 comparing with Control PBS on the same day; # p < 0.05 or ## p < 0.01 comparing with MB + Vitamin C-treated cells on the same day. (C) Comparison of the mitochondrial specific superoxide (MitoSOX) levels in young and old male fibroblasts after the treatment of control PBS, 0.01% DMSO, 100 nM MB, 100 nM Vitamin A, 100 μM Vitamin C, or 100 nM MB + 100 μM Vitamin C for 4 weeks. p < 0.05, **p < 0.01 comparing with Control PBS. (D) Comparison of the final mitochondrial specific superoxide (MitoSOX) levels in young and old female fibroblasts after the treatment of PBS control, 0.01% DMSO, 100 nM MB, 100 nM Vitamin A, 100 μM Vitamin C, and 100 nM MB + 100 μM Vitamin C for 4 weeks. *p < 0.05, **p < 0.01 comparing with Control PBS.