Figure 6 | Scientific Reports

Figure 6

From: Tumor necroptosis is correlated with a favorable immune cell signature and programmed death-ligand 1 expression in cholangiocarcinoma

Figure 6

Tumor necroptosis increases proinflammatory cytokine and chemokine expression. (A) RMCCA-1 cells were treated with TSZ for 8 h. The transcriptional change of TNF-α, IL-1β, CXCL1, CXCL2, CXCL8, CXCL9, CXCL10, CCL3, CCL4, CCL20, MCP-1, ICAM-1, and CSF-1 were measured by qRT-PCR. (B) The time-course analysis of the selected chemokine gene expression, including CXCL2, CXCL8, CCL3, and CCL20 following TSZ treatment in RMCCA-1 (C) The percentage of cell death after stimulation with TSZ for indicated time points using AnnexinV/PI staining and analyzed by flow cytometry. (D) RMCCA-1 cells were treated with TNF-α or SM-164 (S) or SM-164 and zVAD-fmk (SZ) or apoptosis induction (TS). The cells were also pretreated with necrostatin-1 (Nec-1), a necroptosis inhibitor, followed by TSZ treatment for 24 h.

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