Figure 7 | Scientific Reports

Figure 7

From: Tumor necroptosis is correlated with a favorable immune cell signature and programmed death-ligand 1 expression in cholangiocarcinoma

Figure 7

Tumor necroptosis promotes T cell activation to upregulate PD-L1 expression in CCA cells. (A) Western blot analysis for PD-L1 expression of RMCCA-1 and HuCCT-1 treated with IFNγ at 10 and 50 ng/ml for indicated time points. (B) The correlation analysis of CD8+ T cells or CD8+ T cells/Foxp3+ ratio with PD-L1 expression in CCA cells (C) Schematic of treatment conditions, RMCCA-1 cells were treated with DMSO or TSZ for 8 h and conditioned medium (CM) were collected. Jurkat cells were stimulated with the conditioned medium and the conditioned medium from each treatment conditions were collected as shown. (D) Western blot analysis for PD-L1 expression of RMCCA-1 treated with conditioned media from Jurkat.

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