Figure 7
From: Proteomic and genomic analysis of acid dentin lysate with focus on TGF-β signaling
ADL-derived TGF-β activity absorbs to titanium surface and collagen membrane. Titanium discs were soaked in ADL for 1 h followed by three vigorous washes with phosphate buffered saline. Next, gingival fibroblasts were seeded onto the ADL coated titanium discs overnight with and without the inhibitor for the TGF-β RI kinase SB431542. Reverse transcription PCR analysis for (A) IL11 and NOX4 and (B) quantification of IL11 levels by immunoassay. Collagen membranes were treated with ADL for one h followed by three vigorous washes with buffered saline. Then, gingival fibroblasts were seeded onto the ADL-coated collagen membranes overnight. (C) Reverse transcription PCR analysis for IL11 and NOX4. (D) quantification of IL11 levels by immunoassay. N = 4–6. Data represent the mean ± SD. Statistic was based on two-tailed Mann-Whitney and Kruskal–Wallis test, for RT-PCR and immunosorbent assay, respectively.
