Figure 1
From: Regeneration linked miRNA modify tumor phenotype and can enforce multi-lineage growth arrest in vivo
EDU incorporation. (a) Expression of miRNA-23ai/-503i and miRNA-150/-152i in HepG2 cancer cell lines under selection pressure. (i): Fluorescence images of samples transduced with scrambled, miRNA-23ai/-503i and miRNA-150/-152i. (ii): Flow cytometry profiles showing transduced HepG2 cells compared to un-transduced cells. All constructs co-expressed MCherry; bar represents the proportion of positively transduced cells. (iii): Flow cytometry dot plots showing EdU incorporation in HepG2 cells transfected with scrambled, miRNA-23ai/-503i and miRNA-150/-152i. (b) EdU uptake and fold change in Hela, HUH7, Min6, HepG2, RKO, HepaRG and MCF7 cell lines transduced with scrambled, miRNA-23ai, miRNA-503i, miRNA-23ai/-503i, miRNA-150, miRNA-152i and miRNA-150/-152i. The fold change of EDU + /M-Cherry + cells in each cell line are compared against scrambled control vector. Results are representative of three independent experiments. Error bars indicate SD. *p < 0.05,** p < 0.01, ***p < 0.001.
