Figure 3
Sulfated glycosaminoglycans (GAG) are lower and catabolic enzymes are higher within the articular cartilage lining the medial tibial plateau and the medial meniscus from mice after spaceflight (FLIGHT) vs Ground Control (GC) and Vivarium Control (VIV). (a) Representative safranin-O stained sections (n = 5–7/group). (b) Sulfated GAGs in the articular cartilage are lower in FLIGHT vs GC, and vs GC and VIV in meniscus (n = 5–7/group). (c) Representative MMP-13 stained sections (n = 5–7/group). (d) MMP-13 staining was higher in the articular cartilage and menisci in FLIGHT vs GC and VIV (n = 5–7/group). (e) Representative ADAMTS5 stained sections (n = 5–7/group). (f) ADAMTS5 was higher in FLIGHT vs both GC and VIV in articular cartilage (n = 5–7/group). *p < 0.05, **p < 0.01. Scale bar = 100 µm.
