Figure 2 | Scientific Reports

Figure 2

From: G protein-coupled receptor kinase 5 deletion suppresses synovial inflammation in a murine model of collagen antibody-induced arthritis

Figure 2

Effects of GRK5 knockdown by siRNA on NF-κB transcriptional activation and the expression of inflammatory genes in the human synovial SW982 cell line and human primary RA synovial cells. (A) Efficiency of GRK5 knockdown in SW982 cells transfected with small interfering RNA (siRNA)-targeting GRK5 (siGRK5). Values are means ± SD (n = 3). ***P < 0.001 versus siRNA for control (siCtrl). (B) NF-κB transcriptional activation in siGRK5-transfected SW982 cells. Values are means ± SD (n = 4). **P < 0.01 versus siCtrl. (C) Changes in gene expressions in response to LPS stimulation in siGRK5-transfected SW982 cells. Values are means ± SD (n = 3). **P < 0.01; ***P < 0.001. (D) Effect of various inflammatory stimulation on GRK5 gene expression in human primary RA synovial cells. Values are means ± SD (n = 3). (E) Efficiency of GRK5 knockdown in human primary RA synovial cells transfected with siGRK5. Values are means ± SD (n = 4). **P < 0.01. (F) Efficiency of GRK5 inhibition with Amlexanox. Values are means ± SD (n = 4). *P < 0.05; **P < 0.01.

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