Figure 2
From: High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
Yield and purity of EV preparation. Supernatant samples were purified either by the two-step FPLC method or by the conventional DC protocol. (A) Protein recovery from the intermediate SEC step (n = 3) and the final SEC + IMAC (n = 3) and DC (n = 4) products was estimated by a BCA assay and expressed as percentage of the protein content of the starting raw sample. (B) Particle recovery from the intermediate SEC step (n = 2) and the final SEC + IMAC (n = 2) and DC (n = 3) products was estimated with NTA and expressed as percentage of the particle content of the raw sample. (C) We considered the ratio number of particles/µg protein as a measure of purity of the EV preparation. Both the SEC + IMAC (n = 3) and the DC (n = 3) product fall in the range of what are considered pure EV preparations. Each symbol in the graph represents one sample. Data is represented as mean ± s.d. **= p < 0.01.
