Figure 5
From: High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
Analysis of EV markers distribution after centrifugation on a sucrose gradient. EVs isolated by FPLC (n = 2) or DC (n = 3 for FRα, n = 2 for flotillin) were centrifuged on a sucrose gradient, then samples from each density layer were analyzed by WB for the EV markers FRα and flotillin. (A,B) Pixel intensity was calculated with ImageJ. For each protein, all pixels of a sucrose gradient were summed and single bands were expressed as a percentage of the total pixel amount. Data is represented as mean ± s.d. (C) Example of WB of a FPLC-isolated and (D) DC-isolated EVs sample. (E,F) Correlation between FRα and Flotillin distribution across layers was significant for FPLC samples, but not DC samples.
