Figure 2 | Scientific Reports

Figure 2

From: Glycoproteomic analysis of the changes in protein N-glycosylation during neuronal differentiation in human-induced pluripotent stem cells and derived neuronal cells

Figure 2

Immunofluorescence assays using antibodies against differentiation marker proteins. (a) R-10G (left panel), rBC2LCN (middle panel), and SSEA4 (right panel) in the membrane of the iPS cells. (b) Nestin (left) in the cytoplasm, PAX6 (second left) in the nucleus, SOX1 (second right) in the nucleus, and SOX2 (right) in the nucleus of the iPSC-derived neural stem cells. (c) Nestin (left) in the cytoplasm, PAX6 (second left) in the nucleus, SOX1 (second right) in the nucleus, SOX2 (right) in the nucleus in the iPSC-derived neural progenitor cells. (d) Tyrosine hydroxylase (left) in the cytoplasm, FOX2 (middle) in the nucleus, and Tuj1 (right) in the cytoplasm in the iPSC-derived dopamine neurons. The staining was green for different antibodies and blue for 4′,6-diamidino-2-phenylindole (DAPI). Scale bars: 200 μm.

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