Figure 5
CYGB protein expression is upregulated under hypoxic conditions or in presence of PHD inhibitor. Representative immunoblots of CYGB in A375 (A) and Malme-3M (B) cells after 48 h under normoxic (N) or hypoxic (H) (0.1% O2) conditions, in the presence of overexpressed YFP-HIF-1α or YFP-HIF-2α (24 h), and upon treatment with 4 mM PHD inhibitor (PHDi) (24 h). HIF-1α and HIF-2α were revealed using a mouse monoclonal anti-HIF-1α or a rabbit monoclonal anti-HIF-2α antibody, respectively. CYGB was detected with a rabbit polyclonal anti-CYGB antibody. β-actin (ACT) was used as a loading control and revealed using a rabbit monoclonal anti-β-actin antibody.
