Table 2 Reference gene stability.

From: A reliable set of reference genes to normalize oxygen-dependent cytoglobin gene expression levels in melanoma

Rank

Replicate 1

Replicate 2

Replicate 3

GeNorm

NormFinder

GeNorm

NormFinder

GeNorm

NormFinder

1

B2M

B2M

B2M

YWHAZ

YHWAZ

B2M

2

YHWAZ

TBP

YHWAZ

HPRT-1

ACTB

TBP

3

TBP

YWHAZ

TBP

B2M

B2M

YWHAZ

4

UBC

ACTB

UBC

TBP

TBP

ACTB

5

HPRT-1

UBC

SDHA

UBC

UBC

UBC

6

SDHA

HMBS

HPRT-1

SDHA

SDHA

HMBS

7

HMBS

HPRT-1

HMBS

HMBS

HMBS

HPRT-1

8

ACTB

SDHA

ACTB

ACTB

HPRT-1

SDHA

  1. Ranking of selected reference genes based on stability.
  2. Similar to GeNorm, NormFinder is a mathematical algorithm used to identify the best normalization gene according to their expression stability (M)31. Two consistent most stable references genes are labeled in bold.
Back to article page