Figure 2
From: Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866
Changes in the levels of O-GlcNAc/eNOS in HEK293 cells (A) After decreasing PH, changes in the expression of O-GlcNAc/eNOS in HEK293 cells. Typical images and statistical graphs are shown, n = 3. (B) Changes in the levels of O-GlcNAc/eNOS in HEK293 cells under hyperthermic conditions. Typical images and statistical graphs are shown, n = 3. (C) Changes in the levels of O-GlcNAc/eNOS in HEK293 cells treated with hydrogen peroxide. Typical images and statistical graphs are shown, n = 3. (D) Changes in the levels of O-GlcNAc/eNOS in HEK293 cells treated with NaCl (200 mM). Typical images and statistical graphs are shown, Full-length blots are presented in Supplementary Figure S7A, S8B, S9C, S10D. n = 3. *P < 0.05; ns indicates no significance.
