Figure 4
From: Hypertonic stress modulates eNOS function through O-GlcNAc modification at Thr-866
Hypertonic stress increased the levels of eNOS O-GlcNAc and OGT in an AMPK-dependent manner. (A) Changes in the ratio of p-AMPK/AMPK in BAECs treated with NaCl (190 mM). Typical images and statistical graphs are shown, n = 3. (B) Changes in the levels of O-GlcNAc/eNOS in BAECs treated with NaCl (190 mM). Typical images and statistical graphs are shown, n = 3. (C) Changes in the ratio of OGT/GAPDH in BAECs treated with NaCl (190 mM). Typical images and statistical graphs are shown, Full-length blots are presented in Supplementary Figure S15A, S16B, S17C. n = 3. *P < 0.05, ns indicates no significance.
