Figure 6

The burrowing behavior as the weight of the burrowing tunnel in grams for baseline (B) and post-procedure. Weight is shown at 2 h (B 20 and PP 20) and 12 h (B 9 and 24 h PP 8) after the start of the test (B 18 and PP 18) in male and female mice and displayed for each treatment group. Post hoc testing with Bonferroni correction was used to compare burrowing behavior between the treatment groups. Male Pa mice removed significantly more pellets from the burrowing tunnel at B20 compared to LiBupOP mice. At P20, male Pa mice removed significantly more pellets than PaLiBupOP, LiBupOP, and PaOP mice. Male PaAn animals removed significantly more pellets than PaOP animals at P20. In female animals, the Pa group removed significantly more pellets than the LiBupOP, PaOP, and PaAn groups at B20. At P20, female PaLiBupOP mice removed significantly fewer pellets than LiBupOP and Pa mice; LiBupOP mice removed significantly more than PaOP and PaAn mice; Pa mice removed significantly more pellets than PaOP. At 24 h after surgery or anesthesia (PP 8), the PaLiBupOP group removed significantly fewer pellets than the LiBupOP and Pa groups. Significant differences to the respective baseline measurements are indicated with *p ≤ 0.05, Dunnett’s post hoc. Data are shown as scatter dot plot with mean ± SD. Numbers per group: PaLiBupOP, LiBupOP, PaOP and PaAn n = 10/per sex; Pa male n = 10, Pa female n = 8.