Figure 1 | Scientific Reports

Figure 1

From: Novel recombinant keratin degrading subtilisin like serine alkaline protease from Bacillus cereus isolated from marine hydrothermal vent crabs

Figure 1

Protease assay and phylogenetic tree. (a) Protease activity assay by Skim milk assay agar of Bacillus cereus, (b) The evolutionary history was inferred by using the Maximum Likelihood method using MEGA X software. The tree with the highest log likelihood (− 160,829.40) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches, (c) The PCR amplified gene (1050 bps gene amplified by specific primers), (d) The SDS-PAGE analysis of protein expression. Lane M: Protein ladder. Lane C control: uninduced culture. Lane 1–8: The culture incubation time: 1–8 h. A. The bacterial culture induced with 0.5 mM IPTG incubated at 37, (e) Purified Protein. Lane M-Protein marker, Lane 2-Control, lane3–4 Purified protein in buffer 5 with 200 mM and 500 mM imidazole concentration in elution buffer, respectively, (f) Zymography analysis of purified protein with 1% gelatin as substrate.

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