Table 1 Buffers and their composition used in the optimization of expression of recombinant SLSP-k.
S. no. | Buffer | Final concentration |
|---|---|---|
1 | Tris–HCl (Merck), pH 7.5 | 20 mM |
Dithiothreitol (DTT) (Sigma-Aldrich) | 0.1 mM | |
Lysozyme (Sigma-Aldrich) | 1 mg/mL | |
2 | Tris–HCl (Merck), pH 7.5 | 20 mM |
NaCl | 0.5 mM | |
Lysozyme (Sigma-Aldrich) | 1 mg/mL | |
3 | Phosphate buffer | 20 mM |
NaCl | 0.5 mM | |
Urea | 8 M | |
Triton-X 100 | 1% | |
4 | Phosphate buffer | 20 mM |
NaCl | 0.5 mM | |
Triton-X 100 | 1% | |
5 | Phosphate buffer | 20 mM |
Triton-X 100 | 0.5 mM |
