Figure 2
From: Axonopathy precedes cell death in ocular damage mediated by blast exposure
At 5 weeks post bTBI, cell loss from the ganglion cell layer was significant. (a) Images of the retina acquired by spectral domain optical coherence tomography (SD-OCT) at the indicated time points, with the ganglion cell complex (GCC) highlighted by red lines. (b) Representative images from the mid-central area of whole-mount retinas from sham and injured mice at the indicated time points, stained with hematoxylin and eosin (H&E). (c) Thickness of the GCC in vivo, as determined by SD-OCT at 1 week (1 W), 4 weeks (4 W), and 16 weeks (16 W) after bTBI. (d) Density of all cells in ganglion cell layer was quantified in whole-mount retinas stained with H&E at 1 W, 5 W, and 16 W after injury. Bars represent mean ± SD and each data point represents a biological sample; unpaired two tailed t-test analysis with Welch’s correction: *p < 0.05, **p < 0.01, ***p < 0.001, n.s. not significant. The images were processed in Fiji111, the graphs were generated in Prism 8 software and the figure was assembled in Adobe Illustrator CC 2014.
