Figure 5 | Scientific Reports

Figure 5

From: Single tryptophan Y160W mutant of homooligomeric E. coli purine nucleoside phosphorylase implies that dimers forming the hexamer are functionally not equivalent

Figure 5

Thermophoretic titration curves of the enzyme with phosphate (left panel), CD titration curves of the enzyme with phosphate (middle panel), calorimetric titration curves of enzyme-phosphate complex with formycin A (right panel). On each panel data for the WT enzyme (violet) and the Y160W mutant (green) are presented: data (points), global best fit model (lines) and residual plots below. For the sake of clarity the number of data has been limited to one curve from each titration series. Complete data are shown in the Supplementary Information. Titrations were performed at 25 °C, in 50 mM Tris/HCl buffer pH 7.6. For each method and each protein variant the curve with the largest change of the measured physical property was chosen.

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