Figure 6 | Scientific Reports

Figure 6

From: Single tryptophan Y160W mutant of homooligomeric E. coli purine nucleoside phosphorylase implies that dimers forming the hexamer are functionally not equivalent

Figure 6

Thermophoretic titration curves of PNP-Pi complexes with formycin A: WT (left panel) and Y160W (right panel). Data (points), global best fit model (lines) and residual plots are presented. Titrations were performed at 25 °C, in 50 mM Tris/HCl buffer pH 7.6 and with the relative heating laser power at 80% for both WT protein and Y160W mutant. Seven repetitions of each titration were measured.

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