Figure 6
From: Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
Expression of mRNA for adipocyte related genes and intramuscular fat accumulation in WT and Fgf2KO mice. Young (5 m) and old (20–24 m) WT and Fgf2KO mouse hindlimb muscles were isolated and total RNA extracted from whole muscle for qPCR. (a) Plin1 mRNA was significantly increased in old WT and old Fgf2KO relative to young of both genotypes. (b) Pparg2 mRNA, (c) Adiponectin mRNA was significantly increased in old Fgf2KO relative to young Fgf2KO (d) Ap2 mRNA was significantly increased in old WT and old Fgf2KO compared with young of both genotypes. n = 4–8 mice/group. #Compared to corresponding 5 m p < 0.05. (e) AP2/GFP labeling is observed in young and old muscle of both genotypes but markedly increased staining is observed in muscle from old Fgf2KO mice compared with old WT. (f) Oil Red O staining showed markedly increased intramuscular fat tissue (arrows) in 20 m WT and 20 m Fgf2KO compared to 5 m-WT.
