Figure 3 | Scientific Reports

Figure 3

From: AMPK activates Parkin independent autophagy and improves post sepsis immune defense against secondary bacterial lung infections

Figure 3

Parkin signaling and autophagy are engaged in activated macrophages. (a) Bioenergetic profile (OCR/ECAR) in macrophages-treated with LPS (0 or 30 ng/ml) for 24 h. Data presented as mean ± s.d., n = 5 individual measurements per group. (b) Flow cytometry contour plots and quantitative analysis of JC-1 red/green fluorescence in macrophages treated with or without LPS (30 ng/ml) for 30 min. Mean ± s.e.m., n = 3. *P < 0.05, Student’s t-test. (c) Representative images show accumulation of GFP-LC3 fluorescence in control, LPS or FCCP-treated macrophages for 4 h. Areas within dashed boxes are depicted in images on the right side. White arrows indicate cells positive for GFP-LC3 accumulation. GFP-LC3 (green), nuclei (blue). Scale bar 25 µM. (d) Western blot analysis of Parkin in macrophages treated with LPS (0 or 1 µg/ml) or FCCP (0 or 500 nM) for indicated time. Data presented as mean ± s.e.m., n = 5. *P < 0.05, ANOVA.

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