Table 2 Possible tryptic peptides of homicorcin and its variant and their presence in MS analysis.

From: A plant endophyte Staphylococcus hominis strain MBL_AB63 produces a novel lantibiotic, homicorcin and a position one variant

Peptide fragment

Domain

Tryptic peptide

m/z

Presence in MS

Homicorcin/

homicorcin 1

A/A’

N-terminal

S(de)LGTAIK/S(hpo)LGTAIK

653.4/656.8

 + 

B

N-terminal

AT(de)K

301.2

–

C

N-terminal

NACPK

532.2

−

B + C

N-terminal

AT(de)KNACPK

814.4

 + 

D

Hinge

VT(de)R

357.2

 + 

B + C + D

N-terminal + Hinge

AT(de)KNACPKVT(de)R

1153.5

 + 

E

C-terminal

LVT(de)VS(de)CQK

841.5

−

F

C-terminal

S(de)DCQ

434.1

−

D + E

Hinge + C-terminal

VT(de)RLVT(de)VS(de)CQK

1179.7

−

E + F

C-terminal

LVT(de)VS(de)CQKS(de)DCQ

1256.6

 + 

D + E + F

Hinge + C-terminal

VT(de)RLVT(de)VS(de)CQKS(de)DCQ

1594.8

−

  1. A’ denotes fragment A of homicorcin 1 where dehydrated first Ser residue was modified to 2-hydroxypropionate (Hpo). Domain name represents the fragment position in the core peptide. Bold and underlined ‘S(de)’ or ‘T(de)’ residues are predicted to be dehydrated and ‘C’ are predicted to form thio-ether linkage with dehydrated S/T residues. . ( +) and (−) represents the presence or absence respectively of the fragment in MS analysis.
Back to article page