Figure 3
Inhibitory effects of an RAR-α agonist on TGF-β2-induced pro-MMP2 and TIMP-1 expression in RPE cells. (a) RPE cells were cultured in collagen gels with or without TGF-β2 (1 ng/ml) and Am580 (10 µM) for 48 h, after which the culture supernatants were subjected to gelatin zymography for detection of pro-MMP2. (b) Quantitation of relative pro-MMP2 band intensity for gels similar to that in (a). (c) RPE cells cultured as in (a) were lysed and subjected to immunoblot analysis with antibodies to TIMP-1 and to β-tubulin. (d) The intensity of each TIMP-1 band in blots similar to that in (c) was normalized by that of the corresponding β-tubulin band, and the normalized values were expressed relative to that for control cells. Quantitative data in (b) and (d) are means ± s.d. from four independent experiments. The intensity of each bands was measured with the use of the Gels commands in ImageJ software. **P < 0.01 (Dunnett’s test).
