Figure 4
Inhibitory effect of an RAR-α agonist on the TGF-β2-induced expression of paxillin in RPE cells. (a) RPE cells were cultured in collagen gels with or without TGF-β2 (1 ng/ml) and Am580 (10 µM) for 48 h, after which cell lysates were prepared and subjected to immunoblot analysis with antibodies to paxillin and to β-tubulin. (b) The intensity of each paxillin band in blots similar to that in (a) was normalized by that of the corresponding β-tubulin band, and the normalized values were expressed relative to that for control cells and are presented as means ± s.d. for four independent experiments. The intensity of each immunoreactive bands was measured with the use of the Gels commands in ImageJ software. **P < 0.01 (Dunnett’s test).
