Figure 7
Inhibitory effect of an RAR-α agonist on TGF-β2-induced nuclear translocation of MRTF-A in RPE cells. (a) Serum-deprived RPE cells cultured on cover glasses in 24-well plates were incubated with or without Am580 (10 µM) for 12 h and then in the additional absence or presence of TGF-β2 (10 ng/ml) for 24 h, after which they were fixed and subjected to immunofluorescence analysis of MRTF-A (green). Nuclei were stained with DAPI (blue). Scale bars, 20 µm. Data are representative of four independent experiments. (b) The nuclear/cytoplasmic ratio of MRTF-A immunofluorescence intensity in experiments as in (a) was determined and expressed relative to that for control cells. Data are means ± s.d. for a total of 20 cells in four independent experiments. The intensity of MRTF-A immunofluorescence in the nucleus and cytoplasm was measured with the use of the ROI manager commands in ImageJ software. **P < 0.01 (Dunnett’s test).
