Figure 8
Inhibitory effect of an RAR-α agonist in a mouse model of subretinal fibrosis. Intravitreal injection (1 µl) of either PBS vehicle (a) or Am580 (50 µM) (b) was performed both immediately and 3 days after laser photocoagulation. Choroidal flat-mount preparations from the mice at 3 weeks after photocoagulation were subjected to immunofluorescence staining with antibodies to collagen type I (green). Arrowheads indicate photocoagulation-induced subretinal fibrosis. Scale bars, 100 µm. The area of fibrosis was measured with the use of the Measure command in ImageJ software and determined for the treated mice as mean ± s.d. values (n = 40 laser spots per group) (c). *P < 0.05 (Mann-Whitney test).
