Figure 1 | Scientific Reports

Figure 1

From: 5-LO-derived LTB4 plays a key role in MCP-1 expression in HMGB1-exposed VSMCs via a BLTR1 signaling axis

Figure 1

Role of HMGB1 on MCP-1 expression in VSMCs. Rat aortic VSMCs were treated with HMGB1 (0 to 100 ng/ml) for 48 h (a) or HMGB1 (30 ng/ml) for 0 to 48 h (b). The MCP-1 expression levels were determined by Western blotting. β-actin was used as an internal control. Relative intensities were expressed as the mean ± SEM of 3–4 independent experiments. *p < 0.05 and **p < 0.01 vs. corresponding value in control or 0 h. Image analysis was performed using the Amersham-Imager 680, ver. 2.0. software (https://www.cytivalifesciences.com/). Signal was quantified with GraphPad Prism 5, ver. 5.01. software (https://www.graphpad.com/). Cropped gels were displayed and full-length blots/gels are presented in supplementary Fig. S1.

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