Figure 2
Role of HMGB1 on 5-LO activity and expression in VSMCs. VSMCs were treated with HMGB1 (0 to 100 ng/ml) for 12 h or HMGB1 (30 ng/ml) for 0 to 24 h. (a) LTB4 production was measured in the cell culture media, and results were expressed as the mean ± SEM of 3 independent experiments. **p < 0.01 vs. corresponding value in control or 0 h. (b) The 5-LO expression levels were determined by Western blotting. β-actin was used as an internal control. Relative intensities were expressed as the means ± SEMs of 4–5 independent experiments. **p < 0.01 vs. corresponding value in control or 0 h. Image analysis was performed using the Amersham-Imager 680, ver. 2.0. software (https://www.cytivalifesciences.com/). Signal was quantified with GraphPad Prism 5, ver. 5.01. software (https://www.graphpad.com/). Cropped gels were displayed and full-length blots/gels are presented in supplementary Fig. S2.
