Figure 5 | Scientific Reports

Figure 5

From: Probiotic Aspergillus oryzae produces anti-tumor mediator and exerts anti-tumor effects in pancreatic cancer through the p38 MAPK signaling pathway

Figure 5

Heptelidic acid exerted anti-tumor effects mediating the p38 MAPK signaling pathway in pancreatic cancer cells. A Western blotting analysis indicated that the expression of cleaved PARP and cyclin B1 was increased, and that of cyclin D1 was decreased in SUIT-2 cells by treatment with 1 μg/mL of heptelidic acid (A). TUNEL staining showed that the DNA fragmentation of SUIT-2 cells was induced by treatment with 1 μg/mL of heptelidic acid (B). Western blotting showed that the phosphorylation of p38 MAPK was augmented while that of Akt was attenuated by treatment with 1 μg/mL heptelidic acid (C). The SRB assay showed that the growth inhibition rate of heptelidic acid was decreased by treatment with SB203580 (D). The error bars show the standard deviation (S.D.) (A; p-p38; n = 6, p-Akt, p-JNK, p-GSK3β, p-ERK; n = 3) (C,D; n = 3) (B; n = 4).

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