Figure 1
From: ADAM10 and ADAM17 regulate EGFR, c-Met and TNF RI signalling in liver regeneration and fibrosis
Alb-Cre mediated deletion of ADAM10 and ADAM17 genes. (a) Diagram of a genotyping strategy depicting sizes of the PCR bands in respective alleles. (b) Semiquantitative PCR showed almost complete elimination of floxed exons in genomic DNA of primary hepatocytes isolated from ADAM10ΔAlb, ADAM17ΔAlb and ADAM10ΔAlbADAM17ΔAlb mice. Cre negative littermates from ADAM10ΔAlbADAM17ΔAlb were used as controls. (c) Quantitative RT-PCR showed significant reduction of Adam10 and Adam17 mRNA levels in whole liver tissue of ADAM10ΔAlb, ADAM17ΔAlb and ADAM10ΔAlbADAM17ΔAlb mice. Expression was normalized to Gapdh. (n = 5 per group, mean ± SEM, **p < 0.01. Significance was determined by analysis of ΔCt.) (d) ALT and AST levels in serum from unchallenged 32 weeks old mice showed no hepatocellular damage in either of studied lines. (n per group is indicated as a number above the bar, mean ± SEM).
