Figure 4

HGF and TNF RI signalling in ADAM10^ΔAlbADAM17^ΔAlb after hepatectomy. (a) ELISA analysis of mice serum display elevated levels of HGF 6 h post hepatectomy in all studied groups of mice. (n indicated above the bars, mean ± SEM) (b) Shedding of c-Met into serum increases after hepatectomy. Levels of shed c-Met, determined by ELISA, were lower in ADAM10^ΔAlbADAM17^ΔAlb in all studied time points post hepatectomy. (n indicated above the bars, mean ± SEM, *p < 0.05, ***p < 0.001) (c) Phosphorylation of c-Met receptor (pMet) and AKT in primary hepatocytes after 30 min HGF treatment was analysed in lysates by immunoblotting. ADAM10^ΔAlbADAM17^ΔAlb primary hepatocytes show higher phosphorylation of cMet and AKT than control primary hepatocytes. (d) Densitometric evaluation of immunoblots from (c). Representative of three independent experiments shown. (*p < 0.05). (e) Serum levels of sTNF RI, determined by ELISA, were increased after hepatectomy. sTNF RI levels are higher in ADAM10^ΔAlb mice, while ADAM17^ΔAlb and ADAM10^ΔAlbADAM17^ΔAlbshed lower amounts of sTNF RI after hepatectomy. (n indicated above the bars, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001). (f) Levels of shed sTNF RI from primary hepatocytes into culture medium after 6 h of cultivation. (n indicated above the bars, mean ± SEM, *p < 0.05).