Figure 5
From: ADAM10 and ADAM17 regulate EGFR, c-Met and TNF RI signalling in liver regeneration and fibrosis
Model of fibrogenesis induced by CCl4 intoxication. (a,b) Serum levels of ALT and AST from mice after 4-weeks treatment with CCl4 show highest hepatocyte damage in ADAM10ΔAlb mice. (n indicated above the bars, mean ± SEM,*p < 0.05) (c) Sirius red staining shows higher deposition of fibrotic collagens in ADAM10ΔAlb than other studied groups. (d) Quantitative analysis of Sirius red-positive area. (n indicated above the bars, mean ± SEM, **p < 0.01) (e) Ki-67 staining shows than number of proliferating hepatocytes was highest in ADAM10ΔAlb after 4-weeks treatment with CCl4. (f) Quantitative analysis of Ki-67 staining. Only nuclei corresponding to hepatocytes (identified by shape) were counted. (n indicated above the bars, mean ± SEM, *p < 0.05) (g) Immunoblotting of pAKT and total AKT in liver lysates revealed higher activation of AKT pathway in ADAM10ΔAlb mice after CCl4 treatment. (*p < 0.05).
