Figure 2
From: Viral-mediated transduction of auditory neurons with opsins for optical and hybrid activation
ChR2-H134R-EYFP expression in cochlear SGNs following RWM AAV injection in neonatal C57BL/6 mice. (A) The average percentage of EYFP-expressing SGNs was significantly different between each turn (p < 0.05; *p < 0.001 between base and apex; RM ANOVA; n = 10). A similar transduction pattern was observed on the contralateral side, but with overall lower transduction (error bars show the standard error of the mean; one mouse with no ChR2-H134R-EYFP expression was excluded in order to show average expression in successfully transduced mice). (B) Section of an injected cochlea showing the middle and apical turns. Transduced cells were visualised via the EYFP reporter (green) and the neurons were counterstained with the Tuj1 antibody (red). Expression of ChR2-H134R-EYFP was observed in SGN cell bodies and their central and peripheral fibres. (C-E) Images of Rosenthal’s canal from the basal, middle and apical turns of RWM-injected neonatal mice. The proportion of SGNs expressing ChR2-H134R-EYFP increased in a basal to apical direction. (F–H) Contralateral side Rosenthal’s canal images from the basal, middle and apical turns. Scale bar in H applies to images C to H. Minor image processing (brightness) was applied equally to the EYFP channel across C-H for representation purposes.
