Figure 5
From: Viral-mediated transduction of auditory neurons with opsins for optical and hybrid activation
Responses to optical stimulation via a RWM-positioned optical fibre (neonatally AAV-injected C57BL/6 and ChR2-H134R-EYFP transgenic mice) (A) Spatial response images showing multi-unit activity at ventral recording sites in response to 1 ms optical stimulation applied via the RWM of the cochlea in a mouse with 6–40% ChR2-H134R-EYFP expression in the basal-mid turn region. (B) Image of Rosenthal’s canal from the basal turn of the mouse recorded in (A) showing ChR2-H134R-EYFP expression in occasional SGNs. (C) Multi-unit responses to RWM-applied optical stimulation recorded from a mouse with 72–82% of SGNs expressing ChR2-H134R-EYFP in the basal-mid turn region, as shown by the basal turn image of Rosenthal’s canal in (D). (E) Multi-unit responses to optical stimulation in a ChR2-H134R-EYFP transgenic mouse. A second area of activation at the more dorsal recording sites (arrow) can be observed indicating activation of apical SGNs from an optical fibre positioned in the RWM. This image has been reproduced with permission from35. (F) Image of the basal turn region Rosenthal’s canal from the ChR2-H134R-EYFP transgenic mouse with expression of in all SGNs. Scale bar in F applies to all histological images. Response images were generated as per Fig. 4. The dotted lines compare the width of activation at an equivalent level above threshold (d’ = 1 above threshold). Minor image processing (brightness, contrast) was applied to images for representation purposes.
