Table 1 Differential phenotypic characteristics of strains AF52-21T, CM04-06T, and the related species F. prausnitzii ATCC 27768T.

From: Characterization and description of Faecalibacterium butyricigenerans sp. nov. and F. longum sp. nov., isolated from human faeces

Phenotypic features

1

2

3

Growth

Temperature range (optimum) (°C)

20–42 (37)

30–45 (37)

20–42 (37)

pH range

6.0–7.5

5.0–8.0

6.0–7.5

Salt tolerance (%)

1

3

3

Fermentation products

formic acid (mM)

4.86

7.62

18.20

acetic acid (mM)

69.70

44.8

29.67

butyric acid (mM)

15.08

40.03

39.10

lactic acid (mM)

29.25

30.53

5.70

Hydrolysis of

Aesculin

 + 

 − 

 + 

Gelatin

 − 

 + 

 − 

Acid from (API 20A and API 50CHL)

Cellobiose

 + 

 − 

w

D-Fructose

w

 − 

 + 

D-Fucose

w

 − 

w

D-Galactose

w

 − 

 − 

D-Glucose

w

 − 

 + 

D-Lactose

 + 

 − 

 − 

D-Maltose

 + 

 + 

w

D-Mannitol

 + 

 − 

 − 

D-Mannose

 + 

 + 

 − 

D-Raffinose

 − 

w

 − 

D-Trehalose

 + 

w

w

Gluconate

 − 

 − 

 + 

Glycogen

 + 

 − 

 − 

Inositol

w

 − 

 − 

Inulin

 + 

 − 

 + 

Methyl-β-D-Xylopyranoside

w

 − 

 − 

Enzyme activity (API ZYM)

N-acetyl-β-Glucosaminidase

 − 

w

 − 

Naphthol-AS-BI-Phosphohydrolase

 + 

 − 

 + 

α-Glucosidase

 − 

 − 

 + 

β-Galactosidase

 − 

 − 

w

β-Glucosidase

 + 

 − 

 − 

β-Glucuronidase

 + 

w

 + 

DNA G + C (mol %)

57.77

57.51

52 − 57

  1. Strains: 1, F. butyricigenerans AF52-21T; 2, F. longum CM04-06T; 3, F. prausnitzii ATCC 27768T. + , positive; w, weakly positive; −, negative. All data are from this study.