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Figure 1

From: Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)

Figure 1

(a) Process overview of the cascaded continuous cultivation. Reactor 1 is used for biomass formation. Biomass stream is transferred to reactor 2, where recombinant protein production is induced; (b) showing the full factorial design of experiment (DoE) conducted for the factors dilution rate and feed ratio with the responses space–time yield and specific productivity, center point experiments were conducted in triplicates. (1–4 = run1– run 4, SP starpoint, FR feed ratio).

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