Table 1 Prior knowledge on the expression strain BL21(DE3) producing the N-Pro model protein.

From: Cascaded processing enables continuous upstream processing with E. coli BL21(DE3)

Parameter

BL21(DE3) [N-Pro]

Refs.

Maximum specific growth rate—µmax/h

0.38

18

Maximum specific lactose uptake rate—qs,lac,max [g/g/h]

0.23

18

Maximum specific productivity—qp,max [mg/g/h] in fed batch

70 at a qs,gly of 0.4–0.5 g/g/h

40,41

Induction temperature [°C]

31.5

22

Induction pH [−]

6.7

22