Figure 1

The components of the Tetrahymena C1b projection localize throughout the cilium except the cilium tip. (A-B”) and (D–E”) Merged immunofluorescence confocal images of Tetrahymena cells expressing Spef2A-2V5 (A–A”), Adgb-2V5 (B–B”), Cfap69-2V5 (D–D”) or Cfap246-2V5 (E–E”) under the control of the respective native promoters, double stained with anti-V5 (green) and anti-α-tubulin (red) antibodies. (A’, B’, D’, E’) A magnification of cilia marked with white insets in (A, B, D), and (E). (A”, B”, D”, E”) The images of the same areas as in (A’, B’, D’) and (E’) but the signal from a green channel was slightly shifted in respect to a red channel to better visualize that the ciliary tips are devoid of the fusion proteins. (C, F) Western blot analyses of 2V5-tagged fusions in cilia isolated from wild-type (WT) and mutant cells expressing: Spef2A-2V5 (C), Adgb-2V5 (C), Cfap69-2V5 (F) or Cfap246-2V5 (F) detected using anti-V5 antibodies. Band of the protein migrating according to the predicted molecular mass is marked by an asterisk.