Figure 2

Tetrahymena mutant cells with deleted CFAP69 or SPEF2A swim with reduced velocity. (A–G) Swimming paths of wild type (A) and mutant cells: CFAP69-KO (B), CFAP69-KO rescued (C), SPEF2A-coDel (D), SPEF2A-coDel rescued (E), ADGB-KO (F), and CFAP246-coDel (G). All swimming paths were recorded for 3.2 s at RT using video camera. The cells trajectories are indicated by parallel color lines. Bar = 400 µm. (H) Graph showing the comparison of the distances swum by wild-type (WT) and analyzed mutants during 3.2 s. Error bars represent standard deviation. The traveled distance with standard deviation and number of the measured trajectories (N) are indicated at the bottom of the graph. Numbers above the graph indicate % of the distance swum by wild-type cells. Note that ADGB-coDel cells (knockdown) and ADGB-KO cells swam at similar rate. (I–L) Analysis of the cilia length. (I–K) Confocal immunofluorescence images of wild-type (I) and mutant cells (J–K) stained with anti-α-tubulin antibodies to visualize cilia. (L) Graph showing a length of cilia assembled by analyzed cells. n – mean cilium length with standard deviation, N – number of measured cilia. Observed shortening of cilia in both mutants is statistically significant (p < 0.0001, t-test).