Figure 3

Cilia assembled by Tetrahymena CFAP69-KO or SPEF2A-coDel mutants lack C1b projection and exhibit a rotatory movement. (A) Drawings representing subsequent positions of the cilium during the power (red) and recovery stroke (green) of wild-type and mutant cells prepared based on analyzed movies. (B–E) TEM analyses of the ultrastructure of cilia assembled by wild type, CFAP69-KO, SPEF2A-coDel and ADGB-coDel mutants. (B’–E’) The magnified images of the CA of cilia shown in (B–E). Note a lack of a large C1b projection in CFAP69-KO and SPEF2A-coDel mutants. (F) A drawing showing CA and a selected outer doublet with associated complexes. C1, C2 – microtubules of the CA, C1b – projection missing in analyzed mutants, RS – radial spoke, IDA – inner dynein arm, ODA – outer dynein arm, A, B – tubules of the outer doublet. Drawing was prepared based on cryo-ET images of ciliary structures presented in^2,3.