Figure 3
Daphnetin pretreatment inhibited the infiltration of macrophages and neutrophils. Representative images of immunofluorescence detection of the macrophages markers CD11b (A) and the neutrophil marker Ly6G (B) in the lung tissues. Nuclei were counterstained with DAPI. The results for the quantitation of mean fluorescence intensity (MFI) in the red channel are presented. The histograms were created using GraphPad 5 software(GraphPad Prism 5.01 http://www.xdowns.com/soft/xdowns2009.asp?softid=49668&downid=60&id=52443). The quantitative analysis of immunostaining was used by ImageJ software(Image J 2 system software https://imagej.net/Downloads). CON group, l-arginine vehicle + daphnetin vehicle; SAP group, l-arginine + daphnetin vehicle; DAP group, l-arginine vehicle + daphnetin; SAP + DAP group, l-arginine + daphnetin. Data are presented as the mean ± SD. n = 6 /group. **P < 0.01 vs. CON group. ##P < 0.01 versus SAP group, n.s. P > 0.05 versus CON group. Scale bars: 50 μm.
