Figure 5
Daphnetin inhibits activation of the JAK2–STAT3 pathway in the lungs of mice with PALI. (A) Western blot analysis of p-JAK2, JAK, p-STAT3 and STAT3 expression in lung of each group, Bar graph showed the ratio of p-JAK2: total JAK2 protein (B). p-STAT3: total STAT3 protein (C). Full-length blots are presented in Supplementary Figure 2. The data are presented as the means ± SD. The histograms were created using GraphPad 5 software(GraphPad Prism 5.01 http://www.xdowns.com/soft/xdowns2009.asp?softid=49668&downid=60&id=52443). The quantitative analysis of blots was used by ImageJ software(Image J 2 system software https://imagej.net/Downloads). Full-length blots are presented in Supplementary Figure 2. CON group, l-arginine vehicle + daphnetin vehicle; SAP group, l-arginine + daphnetin vehicle; DAP group, l-arginine vehicle + daphnetin; SAP + DAP group, l-arginine + daphnetin. n = 3 /group, data are representative of three independent experiments. **P < 0.01 versus CON group; ##P < 0.01 versus SAP group, n.s.P > 0.05 versus CON group.
