Figure 6
Daphnetin inhibits activation of the JAK2–STAT3 pathway in the lungs of mice with PALI. Immunohistochemical staining of p-JAK2 (A) and p-STAT3 (B) in lung of each group. The magnification of each histological images were × 200. Quantitative analysis of p-JAK2 (C) and p-STAT3 (D). The data are presented as the means ± SD. The histograms were created using GraphPad 5 software (GraphPad Prism 5.01 http://www.xdowns.com/soft/xdowns2009.asp?softid=49668&downid=60&id=52443). The quantitative analysis of immunostaining was used by ImageJ software(Image J 2 system software https://imagej.net/Downloads). CON group, l-arginine vehicle + daphnetin vehicle; SAP group, l-arginine + daphnetin vehicle; DAP group, l-arginine vehicle + daphnetin; SAP + DAP group, l-arginine + daphnetin. **P < 0.01 versus CON group; ##P < 0.01 versus SAP group, n.s. P > 0.05 versus CON group. Scale bars: 50 μm.
