Figure 8
From: Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol
Effect of 17β-estradiol (10–6, 10–8, or 10–10 M) on biofilm formation of Lactobacillus crispatus V4. (A) Biofilm formation of bacteria grown in MRS medium, with or without 17β-estradiol, studied by the crystal violet staining assay. (B) Structure of the Lactobacillus crispatus CIP104459 biofilm formed in “simulating genital tract secretion” (SGTS) medium and visualized by confocal laser scanning microscopy. All figures are represented at the same scale and show top (X/Y) and lateral (X/Z mean thickness) views of the biofilms formed. Effect of 17β-estradiol on the calculated means biofilm biomass (C), thickness (D) and roughness (E). Results are representative of three independent experiments (★p < 0.05).
