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Figure 1

From: Adsorption of nanoparticles suspended in a drop on a leaf surface of Perilla frutescens and their infiltration through stomatal pathway

Figure 1

Adsorption of AuNPs on the abaxial surface of Perilla frutescens by drop deposition method. (a) Experimental schematics of exposing Perilla frutescens leaf to gold nanoparticle (AuNP) suspension. After washing the leaf, 30 μl drops of AuNP suspension are deposited on the abaxial side of the leaf. After exposure for 1–14 days, the leaf and stem are extracted to investigate the absorption of AuNPs through the stomatal pathway by using two-photon excitation microscopy (TPEM) imaging technique, X-ray micro-computed tomography (μCT), and SEM and EDS analysis. (b) Typical SEM image showing the adsorption of AuNPs on the abaxial side of P. frutescens leaf. Inset shows the magnified stoma in the yellow box. Aggregates of AuNPs are shown in the red box. Scale bar is 100 nm. (c) SEM images showing the magnified stoma (left) and maximum length scale (lmax) of AuNPs (right). (d) Variations in the number of aggregates (Naggregate) (black triangles) and maximum length scale (lmax) (red squares) with respect to the number of particles per aggregate (Nparticle) in the region near the stoma in (c). Error bars indicate standard deviations (SD).

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