Figure 6

Western bolt analysis of metastatic LS-174T cells treated with or without ONC201, at varying concentrations. Differential expression patterns of different proteins that are involved in CHOP regulation and ER stress are shown. Densitometric analysis of the representative Western blots is shown (mean ± SD), with respect to housekeeping gene β-actin in different treatment groups (n = 3). Significant values were set as *P < 0.01. The software ImageJ V1.49O (https://imagej.nih.gov/ij/) was used to quantify the immunoblot signals as a mean of the grey/white scale of all the pixels in a band.