Figure 8

Alternative spliced variants of CHOP. (A) A computational analysis detecting CHOP differential alternative splicing events obtained from the microarray study of SW480 treated with and without ONC201. (B) Bioanalyzer electrophoresis after quantitative PCR analysis of LS-174T and SW480 cells treated with and without ONC201. CHOP was amplified using specific primers flanking the exons 1 and 4. PCR products were fractionated on Bioanalyzer microgel. Alternative spliced isoforms were mapped according to their fragment size.