Figure 5 | Scientific Reports

Figure 5

From: Comprehensive profiling of novel epithelial–mesenchymal transition mediators and their clinical significance in colorectal cancer

Figure 5

Involvement of ARC in TGF-β-mediated EMT pathway. (a) Association between ARC expression and upregulated genes in a panel of epithelial cell lines by TGF-β1 in GSEA (NES = − 1.499, q = 0.130). (b) Alteration of ARC expression in response to TGF-β1 in western blot analysis. Full-length blots are presented in Supplementary Fig. 11d. (c) Alteration of CDH1 expression in sh-NC and sh-ARC SW480 cell lines in response to TGF-β1 in qPCR and alteration of CDH1 and ZEB1 expression in sh-NC and sh-ARC SW480 cells in response to TGF-β1 in western blot analysis. Error bars show standard errors of the mean. Asterisks denote significant difference using unpaired 2-tail t-test (*p < 0.05, **p < 0.01). Full-length blots are presented in Supplementary Fig. 111. (d) TGF-β1-induced change in cell shape in sh-NC and sh-ARC SW480 cell lines. Scale bars indicate 50 µm. Experiments were conducted in triplicate. TGF transforming growth factor, EMT epithelial–mesenchymal transition, GSEA gene set enrichment analysis, NES normalized enrichment score, sh short hairpin, NC negative control, qPCR quantitative polymerase chain reaction.

Back to article page